Amulyaratna Behera

The aim of the present work was to develop and validate a simple RP-HPLC method for the determination of Rebamipide. Chromatographic separation of Rebamipide was achieved by using a C18 column. A Mobile phase containing a mixture of methanol: water (70:30 v/v) was pumped at the flow rate of 1 ml/min. Detection was performed at 231 nm. Validation parameters were evaluated according to the International Conference on Harmonization (ICH) Q2R1 guidelines. The calibration curve was linear in the concentration range 2- 10μg/ml for the Rebamipide with regression coefficient 0.999. The method was found to be accurate with 0.66 % RSD. RSD values were found to be 0.94 % in the case of intra-day precision studies, whereas0.76% in the case of inter-day precision the limits of detection and quantification were found to be 0.6, 1.98μg/ml, respectively. The results obtained from the stress testing reveals that Rebamipide drug substance is particularly stable under acidic, neutral and thermal degradation conditions. From the stability point of view, care should be taken in the manufacturing process and storage of this product

The aim of the present research work was to develop and formulate a bilayer floating sustained release tablet of Nifedipine. Nifedipine is an antihypertensive agent. Surface solid dispersion of Nifedipine was prepared by solvent evaporation method with different super disintegrants as a polymer for improvement of solubility resulting in improved bioavailability. Bilayer consists of two layers, immediate-release layer, and second sustained release layer, compressed in the single unit dosage form. The immediate-release layer contains surface solid dispersion of Nifedipine. The floating sustained release layer also contains surface solid dispersion of Nifedipine, HPMC K100M & HPMC K15M as sustained release polymer. The floating properties of the tablets were achieved by the use of sodium bicarbonate & citric acid which generate the gas upon contact with gastric fluid. The immediate-release layer releases the drug immediately and starts the onset of action and the floating sustained release layer floats on gastric fluid for upto12 hours and releases the drug in a sustained manner, subsequently, it prolongs the duration of action.

The current study is based on Evaluation of Analgesic Activity of Datura Stramonium leaves in Albino Rats. Datura stramonium is belongs to the family solanaceae is mostly found throughout the globe. The hydro alcoholic extracts are used traditionally as a potent analgesic during surgery or bone setting. The aim of the present investigation is to evaluate the analgesic activity of hydro alcoholic leaf extract of Datura stramonium in albino rats. The experiment was performed by inducing Datura stramonium extracts in two different concentrations to test groups by using hot plate method for analgesic study and compared with Tramadol HCL at 40 mg/Kg IP body weight intraperitoneally as reference drugs. The results of hot plate method indicates a dose dependent effect for both the doses of Datura stramonium resembling that of Tramadol HCL in their anti nociceptive effect versus time indicating that the extract have a central analgesic effect probably by both narcotic and non narcotic mechanisms which may be due to various phytochemical contents present in the leaf of the plant Datura stramonium.

The aim of the present study is to formulate & evaluate a suitable matrix type transdermal polymeric film of Olmesartan Medoxomil using two different polymeric combinations, Eudragit RL 100 (E RL 100) and Hydroxyl Propyl Methyl Cellulose (HPMC). The preformulation parameters were evaluated for their suitability to formulate transdermal polymeric film. The transdermal polymeric film was successfully prepared and critically investigated. The evaluation parameter such as FTIR for its compatibility of the drug with polymer, Scanning Electron Microscopy (SEM) photographs of the prepared film were taken to see the drug distribution pattern, Physical studies including moisture content, moisture uptake, flatness to study the stability of the formulations and In vitro permeation study is conducted in a modified Franz's diffusion cell by using rat skin membrane & cellophane membrane. The obtained result confirms that it complies as per specification or reported methods. Based on the above observations, it can be reasonably concluded that E RL 100 & HPMC polymers are the best-suited polymers for the development of TDDS of Olmesartan Medoxomil.

Various types of plant, fruits gums available like alginic acid, gelatin maize starch and Potato starch have been used as binder in pharmaceutical formulation. But still finding novel binder is useful in the pharmaceutical industry for manufacture of tablets. Cordia dichotoma fruit pulp was found for its binding property. Aspirin & ibuprofen tablets were prepared by wet granulation technique using cordia dichotoma fruit pulp as a tablet binder. The prepared tablets were evaluated for physiochemical characteristics and the binding efficacy of the cordia dichotoma fruit pulp was compared with the standard binder gum polyvinyl pyrrolidine (PVP) at similar concentration (3% w/w), 21.04o to 32.02o angle of repose and 0.980.26%w/w friability1.2 to12.03 min disintegration time. Tablets at 3% w/w binder concentration showed more optimum results as tablet binder. Cordia dichotoma fruit pulp was found to be useful for the preparation of uncoated tablet dosage form.