Binod Kumar

This book serves as a hands-on guide to foundational laboratory techniques in veterinary parasitology and molecular biology, specifically tailored for students, interns, and early-career researchers entering lab-based veterinary diagnostics. Divided into three focused chapters, the book begins with essential laboratory practices, including correct lab conduct, familiarization with basic lab equipment, and the preparation of core reagents and solutions necessary for routine experiments. The second chapter introduces key techniques in veterinary parasitology, such as proper sample collection and preservation, blood and fecal parasite detection, and the basics of serum biochemistry and immunodiagnostic assays. The third chapter provides a sequential understanding of molecular biology procedures relevant to parasitology researchranging from DNA extraction and primer design to PCR amplification and sequence analysis. In addition to theoretical instruction, the manual includes a practical appendix for equipment care, troubleshooting, and routine maintenanceensuring efficiency and accuracy in day-to-day laboratory operations. This concise and structured resource supports skill development and diagnostic competence in modern parasitological and molecular veterinary laboratories.

"See, Raman. I'm a twenty-first century girl. The institute of marriage and its duties are now outdated. You earn, and I'm not a house-wife. So, why should my desire to be limited to yours? I believe in an independent life. You, live in your way, and let me live in mine," replied she confidently. "No, Raman, no. You should have had faith in your love. Heart is not a garden in which spring comes every year. It comes only once in it, and gives the happiness the whole life," said Mrs. Malhotra staring at him, but he dared not look into her eyes. "I was like a bird that flies in the sky to touch the heaven, sans thinking it will have to come on earth some day," said she sadly. "Vinita, life is like walking on rope; if you lose your balance, your fall is sure, and the fall may be so heavy that you will never stand up for walking ahead." "May God grant her wish But, when life is given by God, how can man decide its destiny?"