Nandini Manjunath

The present book is initiated to compare the ability of severaldetection methods, i.e., a serial dilution anaerobic culture and/ormicroscopic procedure, a DNA probe procedure and immunologicalreagents using both an enzyme-linked immunosorbent assay (ELISA)and an indirect immunofluorescence assay (IFA), to detect a selectedpanel of periodontopathic organisms in single subgingival plaque samplestaken from periodontally diseased sites.

The red complex is a group of bacteria that are categorized together based on their association with severe forms of periodontal disease. The red complex-among a number of other complexes-were classified by Sigmund Socransky in 1998. The three members of the red complex are: Porphyromonas gingivalis, Tannerella forsythia Treponema denticola . There presence in the oral cavity causes changes in hydrogen ion concentration