Shyamal K Goswami

Fundamentals of Redox Biology provides a complete introduction to the cellular redox systems, exploring their regulation, physiological role and significance to human diseases. The book begins with a historical overview before exploring the fundamental characteristics and functions of free radicals and reactive oxygen species. Their role in redox signaling and epigenetic regulation are covered, as well as the impact of antioxidants on redox homeostasis. Additionally, the book explores how ROS can lead to oxidative stress and various disease states, including cancer, cardiovascular and neurological disorders, while also considering the role of mitochondrial ROS in the immune response against various infections. Biology of cellular redox systems has now become an integral part of many disciplines across the life sciences. This book provides a foundational overview of the topic which will be useful to those in the field of biochemistry, cell biology, molecular biology, immunology, and related areas.

Protocols in Biochemistry and Clinical Biochemistry, second edition, offers clear, applied instruction in fundamental biochemistry methods and protocols, from buffer preparation to nucleic acid purification, protein, lipid, carbohydrate, and enzyme testing, and clinical testing of vitamins, glucose, and cholesterol levels, among other diagnostics. Each protocol is illustrated with step-by-step instructions, labeled diagrams, and color images, as well as a thorough overview of materials and equipment, precursor techniques, safety considerations and standards, analysis and statistics, alternative methods, and troubleshooting, all to support a range of study types and clinical diagnostics. This fully revised edition has been expanded and enriched to feature 100 protocols, as well as chapter key term definitions and worked examples. All-new protocols added to this edition include identification of lipids by TLC, lipid per oxidation measurement by thiobarbituric acid assays, determination of serum amylase, catalase activity assay, superoxide dismutase assay, qualitative analysis of plant secondary metabolites, qualitative analysis of photochemicals, quantitative estimation of secondary metabolites, estimation of chlorophyll contents, and starch determination, among others. Each protocol is written to help researchers and clinicians easily reproduce lab methods and ensure accurate test results.

Protocols in Biochemistry and Clinical Biochemistry offers clear, applied instruction to fundamental biochemistry methods and protocols, from buffer preparation to nucleic acid purification, protein, lipid, carbohydrate, and enzyme testing, and clinical testing of vitamins, glucose and cholesterol levels, among other diagnostics. Each protocol is illustrated with step-by-step instructions, labeled diagrams, and color images, as well as a thorough overview of materials and equipment, precursor techniques, safety considerations and standards, analysis and statistics, alternative methods and troubleshooting.